Carol Cort, owner of Barn Nursery School and Day Nursery in Cheltenham, was charged by Gloucestershire Police on 21 March for assault, ill treatment, neglect/abandoning a child or young person to cause unnecessary suffering or injury.
A spokesperson for Gloucestershire Constabulary has confirmed that Ms Cort is due to appear before Cheltenham Magistrates Court on 2 May.
Ofsted inspectors visited the nursery on Friday to suspend its registration, pending an investigation.
Reports suggest that the nursery was closed on 26 February following a serious incident at the setting the week before. It is believed that the nursery then re-opened before inspectors visited last week.
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