Knightsbridge Kindergarten, a private nursery in Westminster, called in the Health Protection Agency after an adult linked to the nursery was diagnosed with TB in July.
The disease is very difficult to diagnose and it can take several months for symptoms to appear.
Results from screening of all children and staff show that seven children and one further member of staff had an active infection. All are being treated with a six-month course of antibiotics.
Another 26 children and six adults linked to the nursery were found to have TB in their bodies but had no active infection. They will receive a shorter course of treatment.
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