The chain has introduced its own E.coli policy and said it will close any nurseries where a case is suspected to stop it spreading.
On Monday, NHS Fife confirmed the number of cases linked to the nursery as 13.
It is understood that Careshare was informed of the first E.coli case on 5 May. Information from NHS Fife received by the nursery stated, 'It is not thought that the infection came from the nursery or that anyone else at the nursery is likely to be at risk.'
Officials did not advise the nursery to close until 9 May after a second case was confirmed.
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